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The cholinergic neurons inside the PPN and LDT regulate the two SNc and VTA neurons as a result of activating acetylcholine receptors in these neurons [eight]. Combining optogenetic and brain slice patch-clamp tactics, Xiao et al. [8] demonstrated that five–ten?s of optogenetic stimulation of cholinergic projections in the PPN and LDT evokes inwa

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